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- Title
KOLORİMETRİK LOOP-MEDIATED İZOTERMAL AMPLİFİKASYON METODU İLE LISTERIA MONOCYTOGENES'İN TAVUK ETLERİNDE HIZLI TESPİTİ.
- Authors
Yüksel, Mehmet; Sert, Selahattin; Yüksel, Arzu Kavaz; Çetin, Bülent; Gürses, Mustafa
- Abstract
The object of this study is to evaluate the performance of the colorimetric loop-mediated isothermal amplification (LAMP) in detecting Listeria monocytogenes from chicken meats (n: 50; whole wing, breast, drumstick; totally N: 150). For this purpose, the chicken meats were artificially contaminated with 100-104 CFU/25 g (or sample) of L. monocytogenes and 5 others bacteria were used as competitive microbiota. After pre-enrichment, the samples were analyzed using conventional cultural, LAMP and real-time PCR methods. Primer sets of virulence gene hlyA were used for the L. monocytogenes-specific visualized LAMP with hydroxynaphthol blue (HNB) dye and amplified the target gene using specific primers at 65 °C for 45 min. As a result of analysis performed by three methods in the naturally contaminated samples, the presence of L. monocytogenes was detected in 9 of 150 samples (6%). The LAMP, qPCR and conventional method showed similar detection performances for L. monocytogenes in the naturally or artifically contaminated samples. These results demonstrated the use of HNB-LAMP method for, as an alternative to PCR, sensitive and specific to L. monocytogenes under isothermal conditions, could use as a simple, rapid detection technique and has the potential for detection of L. monocytogenes from the chicken meats.
- Subjects
LAMPS; CHICKEN as food; MEAT; BACTERIA; GENES
- Publication
GIDA: The Journal of Food, 2022, Vol 47, Issue 1, p121
- ISSN
1300-3070
- Publication type
Article
- DOI
10.15237/gida.GD21125