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- Title
miR-152-3p Represses the Proliferation of the Thymic Epithelial Cells by Targeting Smad2.
- Authors
Li, Ying; Wang, Xintong; Wu, Qingru; Liu, Fenfen; Yang, Lin; Gong, Bishuang; Zhang, Kaizhao; Ma, Yongjiang; Li, Yugu
- Abstract
MicroRNAs (miRNAs) control the proliferation of thymic epithelial cells (TECs) for thymic involution. Previous studies have shown that expression levels of miR-152-3p were significantly increased in the thymus and TECs during the involution of the mouse thymus. However, the possible function and potential molecular mechanism of miR-152-3p remains unclear. This study identified that the overexpression of miR-152-3p can inhibit, while the inhibition of miR-152-3p can promote, the proliferation of murine medullary thymic epithelial cell line 1 (MTEC1) cells. Moreover, miR-152-3p expression was quantitatively analyzed to negatively regulate Smad2, and the Smad2 gene was found to be a direct target of miR-152-3p, using the luciferase reporter assay. Importantly, silencing Smad2 was found to block the G1 phase of cells and inhibit the cell cycle, which was consistent with the overexpression of miR-152-3p. Furthermore, co-transfection studies of siRNA–Smad2 (siSmad2) and the miR-152-3p mimic further established that miR-152-3p inhibited the proliferation of MTEC1 cells by targeting Smad2 and reducing the expression of Smad2. Taken together, this study proved miR-152-3p to be an important molecule that regulates the proliferation of TECs and therefore provides a new reference for delaying thymus involution and thymus regeneration.
- Subjects
SMAD proteins; EPITHELIAL cells; INHIBITION of cellular proliferation; CELL cycle
- Publication
Genes, 2022, Vol 13, Issue 4, p576
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes13040576