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- Title
Expression Profile of Fatty Acid Metabolism Genes in Preimplantation Blastocysts of Obese and Non-Obese Mice.
- Authors
Königsdorf, Cornelia Alice Irene; Navarrete Santos, Anne; Schmidt, Juliane-Susanne; Fischer, Sünje; Fischer, Bernd
- Abstract
Background: Recent studies have disclosed a close relationship between maternal obesity, fetal metabolism and pre- and postnatal development. The lipid metabolism in preimplantation embryos is a possible target of metabolic programming. Methods: 31 genes of beta-oxidation and fatty acid and cholesterol uptake, synthesis and regulation were analyzed in day 3.5 blastocysts from NZO (obese) and C57Bl/6 (normal weight) mice by RT-PCR and semiquantitative PCR. Results: The most obvious difference between both strains was the lack of the RXR gamma transcript in NZO blastocysts. In adult NZO mice, RXR gamma is detectable in most tissues. In a semiquantitative analysis, a higher transcription rate of fatty acid transport protein 4 (p = 0.004) and a reduced transcript number of fatty acid synthase (p = 0.049) was found in NZO blastocysts. Cholesterol synthesis regulation was modified in NZO blastocysts, as indicated by the ratio of sterol regulatory element-binding protein (SREBP) 2 / insulin-induced gene 1 (Insig 1) (p = 0.001). Conclusion: In mouse blastocysts enzymes and signal molecules of fatty acid and cholesterol metabolism resemble those expressed postnatally. Distinct differences in transcription rates of genes between blastocysts from obese and non-obese mothers indicate that preimplantation embryo development is an early target for metabolic programming. Copyright © 2012 S. Karger AG, Basel
- Publication
Obesity Facts: The European Journal of Obesity, 2012, Vol 5, Issue 4, p575
- ISSN
1662-4025
- Publication type
Article
- DOI
10.1159/000342583