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- Title
Enzyme inhibitory antibody to pyruvate dehydrogenase: diagnostic utility in primary biliary cirrhosis.
- Authors
Jois, Jennifer; Omagari, Katsuhisa; Rowley, Merrill J.; Anderson, John; Mackay, Ian R.
- Abstract
In primary biliary cirrhosis, autoantibodies are produced to the family of 2-oxoacid dehydrogenase complexes. These 'anti-mitochondrial' antibodies are traditionally detected by immunofluorescence but this method of detection is subjective and labour-intensive. We assessed an enzymatic mitochondrial antibody (EMA) assay based on antibody inhibition of enzymatic activity of pyruvate dehydrogenase complex in wells of microtitre plates with a colorimetric read-out. We tested 48 Australian and 1947 Japanese patients with primary biliary cirrhosis, 306 normal subjects and 691 patients with various hepatic and non-hepatic diseases. The overall sensitivity of the EMA for the diagnosis of primary biliary cirrhosis, 82%, was slightly lower than that of immunofluorescence, 90% . The advantages of the EMA test include high specificity, > 99% , and semi-automated features facilitating objectivity, rapidity, simplicity and economy. The EMA test could be particularly applicable to population screening for early primary biliary cirrhosis.
- Subjects
ENZYMES; IMMUNOGLOBULINS; DEHYDROGENASES; CIRRHOSIS of the liver; PYRUVATES
- Publication
Annals of Clinical Biochemistry, 2000, Vol 37, Issue 1, p67
- ISSN
0004-5632
- Publication type
Article
- DOI
10.1258/0004563001901542