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- Title
A fluorometric aptamer nanoprobe for alpha-fetoprotein by exploiting the FRET between 5-carboxyfluorescein and palladium nanoparticles.
- Authors
Li, Guiyin; Zeng, Junxiang; Liu, Huiling; Ding, Ping; Liang, Jintao; Nie, Xinmin; Zhou, Zhide
- Abstract
Alpha-fetoprotein (AFP) is a reliable clinical marker of hepatocellular carcinoma (HCC). A highly sensitive fluorometric aptamer nanoprobe is described for AFP detection. It is based on fluorescence resonance energy transfer (FRET) between AFP aptamer labelled with 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). The PdNPs quench the green fluorescence of the FAM-AFP aptamer via interactions between nitrogen functional groups of the AFP aptamer and PdNPs. When AFP was introduced into the FAM-AFP aptamer-PdNPs FRET system, the AFP aptamer preferentially combines with AFP. This results in a conformational change and weakens the interaction between the aptamer and the PdNPs. Thus, the fluorescence of FAM recovers. The fluorescence recovery of FAM increases linearly in the 5.0–150 ng·mL−1 AFP concentration range and has a 1.4 ng·mL−1 detection limit. The assay was applied to the analysis of spiked diluted human serum. The recovery values ranged from 98.3 to 112.9%, with relative standard deviations of <1.1%. This biosensing strategy provides a reliable and ultrasensitive protocol for the quantification of biomarkers with relevant antigens and aptamers.
- Subjects
ALPHA fetoproteins; FLUORESCENCE resonance energy transfer; PALLADIUM; BIOMARKERS; FUNCTIONAL groups
- Publication
Microchimica Acta, 2019, Vol 186, Issue 5, pN.PAG
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-019-3403-z