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- Title
Fluorescent turn-on determination of the activity of peptidases using peptide templated gold nanoclusters.
- Authors
Luo, Junjun; Rasooly, Avraham; Wang, Liqiang; Zeng, Ke; Shen, Congcong; Qian, Pin; Yang, Minghui; Qu, Fengli
- Abstract
The fluorescence intensity of gold nanoclusters (AuNCs) is inversely related to the length of a peptide immobilized on its surface. This finding has been exploited to design a turn-on fluorescent method for the determination of the activity of peptidase. The β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) was chosen as a model peptidase. BACE1 cleaves the peptide substrates on AuNCs, and the fluorescence intensity of the AuNCs (at exCitation/emission wavelengths of 320/405 nm) carrying the rest of the cleaved peptide is significantly higher than that of the AuNCs with uncleaved peptide. Transmission electron microscopy revealed a decrease in the size of the AuNCs which is assumed cause fluorescence enhancement. The assay was applied to the determination of BACE1 activity in spiked cell lysates, and recoveries were between 96.9 and 104.0 %. [Figure not available: see fulltext.]
- Subjects
PEPTIDASE; GOLD nanoparticles; FLUORIMETRY; CHEMICAL templates; MICROCLUSTERS; AMYLOID beta-protein precursor
- Publication
Microchimica Acta, 2016, Vol 183, Issue 2, p605
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-015-1683-5