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- Title
A dual-strategy expression screen for candidate connectivity labels in the developing thalamus.
- Authors
Bibollet-Bahena, Olivia; Tatsuya Okafuji; Hokamp, Karsten; Tear, Guy; Mitchell, Kevin J.
- Abstract
The thalamus or “inner chamber” of the brain is divided into ~30 discrete nuclei, with highly specific patterns of afferent and efferent connectivity. To identify genes that may direct these patterns of connectivity, we used two strategies. First, we used a bioinformatics pipe- line to survey the predicted proteomes of nematode, fruitfly, mouse and human for extracel- lular proteins containing any of a list of motifs found in known guidance or connectivity molecules. Second, we performed clustering analyses on the Allen Developing Mouse Brain Atlas data to identify genes encoding surface proteins expressed with temporal profiles simi- lar to known guidance or connectivity molecules. In both cases, we then screened the resul- tant genes for selective expression patterns in the developing thalamus. These approaches identified 82 candidate connectivity labels in the developing thalamus. These molecules include many members of the Ephrin, Eph-recept or, cadherin, protocadherin, semaphorin, plexin, Odz/teneurin, Neto, cerebellin, calsyntenin and Netrin-G families, as well as diverse members of the immunoglobulin (Ig) and leucine-rich receptor (LRR) superfamilies , receptor tyrosine kinases and phosphatases, a variety of growth factors and receptors, and a large number of miscellaneous membrane-asso ciated or secreted proteins not previously impli- cated in axonal guidance or neuronal connectivity. The diversity of their expression patterns indicates that thalamic nuclei are highly differentiated from each other, with each one dis- playing a unique repertoire of these molecules, consistent with a combinatorial logic to the specification of thalamic connectivity.
- Publication
PLoS ONE, 2017, Vol 12, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0177977