We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Selection of exopolysaccharide-producing lactic acid bacteria isolates from Inner Mongolian traditional yoghurt.
- Authors
Zhang Chun-lei; Li Jia-qi; Guo Hai-tao; Wang Jie; Xu Ri-hua
- Abstract
Lactic acid bacteria (LAB) isolated from Inner Mongolian traditional yoghurt were evaluated for the production of exopolysaccharides (EPS) by phenol-sulphuric acid method after ethanol precipita-tion and dialysis. Total polysaccharide was extracted from sucrose-containing MRS broth cultures of the selected LAB strains. Comparison of the EPS yields revealed that among tested LAB, strain 37 exhibited the highest production of 536.904 mg/L. The strain was identified as Leuconostoc citreum with carbohydrate assimilation profiling, 16S rRNA and pheS gene sequencing. The Ln. citreum 37 was found to be a novel EPS producing strain. It was found that there was no direct linear relation between the colony size and EPS yield, so the colony size could not to be used to screen EPS-producing strains.
- Subjects
MONGOLIA; MICROBIAL exopolysaccharides; LACTIC acid bacteria; YOGURT; SULFURIC acid; PRECIPITATION (Chemistry)
- Publication
Dairy / Mljekarstvo, 2014, Vol 64, Issue 4, p254
- ISSN
0026-704X
- Publication type
Article
- DOI
10.15567/mljekarstvo.2014.0404