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- Title
Bacterium-Expressed ds RNA Downregulates Microsporidia Nosema bombycis Gene Expression.
- Authors
Pan, Qiuling; Wang, Ling; Dang, Xiaoqun; Ma, Zhengang; Zhang, Xiaoyan; Chen, Shiliang; Zhou, Zeyang; Xu, Jinshan
- Abstract
The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain ( DE3) to express double-strand RNAs targeting the gene encoding ADP/ ATP protein in N. bombycis. The results showed that ds RNAs deferentially suppressed the gene expression during N. bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the ds RNA expressed by recombinant E. coli to control the pebrine disease of the domestic silkworm.
- Subjects
NOSEMA bombycis; PEBRINE; ESCHERICHIA coli; GENE expression; RNA interference; SILKWORM diseases
- Publication
Journal of Eukaryotic Microbiology, 2017, Vol 64, Issue 2, p278
- ISSN
1066-5234
- Publication type
Article
- DOI
10.1111/jeu.12346