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- Title
Klebsiella pneumoniae FimK Promotes Virulence in Murine Pneumonia.
- Authors
Rosen, David A.; Hilliard, Julia K.; Tiemann, Kristin M.; Todd, Elizabeth M.; Celeste Morley, S.; Hunstad, David A.; Morley, S Celeste
- Abstract
Klebsiella pneumoniae, a chief cause of nosocomial pneumonia, is a versatile and commonly multidrug-resistant human pathogen for which further insight into pathogenesis is needed. We show that the pilus regulatory gene fimK promotes the virulence of K. pneumoniae strain TOP52 in murine pneumonia. This contrasts with the attenuating effect of fimK on urinary tract virulence, illustrating that a single factor may exert opposing effects on pathogenesis in distinct host niches. Loss of fimK in TOP52 pneumonia was associated with diminished lung bacterial burden, limited innate responses within the lung, and improved host survival. FimK expression was shown to promote serum resistance, capsule production, and protection from phagocytosis by host immune cells. Finally, while the widely used K. pneumoniae model strain 43816 produces rapid dissemination and death in mice, TOP52 caused largely localized pneumonia with limited lethality, thereby providing an alternative tool for studying K. pneumoniae pathogenesis and control within the lung.
- Subjects
KLEBSIELLA pneumoniae; MICROBIAL virulence -- Immunological aspects; PNEUMONIA; REGULATOR genes; URINARY tract infections; GENE expression; IMMUNOLOGY; LUNG microbiology; TOXIN metabolism; ANIMAL experimentation; BACTERIAL growth; BIOLOGICAL models; IMMUNITY; KLEBSIELLA; MICE; MICROBIOLOGICAL techniques; GENETIC mutation; PHAGOCYTOSIS; RESEARCH funding; SURVIVAL analysis (Biometry); TOXINS; MICROBIAL virulence; BACTERIAL capsules
- Publication
Journal of Infectious Diseases, 2016, Vol 213, Issue 4, p649
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiv440