We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse.
- Authors
Kai Li; Xuedong Wei; Guangbo Zhang; Miao Li; Xuefeng Zhang; Chenhao Zhou; Jianquan Hou; Hexing Yuan; Li, Kai; Wei, Xuedong; Zhang, Guangbo; Li, Miao; Zhang, Xuefeng; Zhou, Chenhao; Hou, Jianquan; Yuan, Hexing
- Abstract
<bold>Background: </bold>B7-H3, a member of the B7 family of the Ig superfamily of proteins, has been detected in the epididymis, which is a storage organ related to sperm maturation. However, the characteristics of its expression in different regions of the epididymis remain unknown. Our aim was to investigate the expression of B7-H3 in the caput, corpus, and cauda of the epididymis.<bold>Methods: </bold>We extracted epididymis specimens from adult male C57BL/6 mice. The expression of B7-H3 was then measured with immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and western blotting.<bold>Results: </bold>B7-H3 protein was predominantly detected on the membrane and in the cytoplasm of the principal cells in the epididymis. Moreover, the level of B7-H3 in the corpus of the mouse epididymis was significantly higher than that in the caput (p < 0.05) or the cauda of the epididymis (P < 0.05). However, there was no remarkable difference in the level of B7-H3 between the caput and the cauda (p > 0.05).<bold>Conclusions: </bold>The caput, corpus, and cauda of the mouse epididymis all expressed B7-H3 protein. However, the levels of B7-H3 were different in the three regions of the epididymis.
- Subjects
HUMAN fertility; SPERMATOZOA; LABORATORY mice; PROSTATE; TESTIS; EPIDIDYMIS; ANTIGEN analysis; ANIMALS; ANTIGENS; MICE; ANATOMY
- Publication
BMC Urology, 2017, Vol 17, p1
- ISSN
1471-2490
- Publication type
journal article
- DOI
10.1186/s12894-017-0215-5