We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Determination of soyasaponins in <italic>Fagioli di Sarconi</italic> beans (<italic>Phaseolus vulgaris</italic> L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity.
- Authors
Bianco, Giuliana; Pascale, Raffaella; Carbone, Cecilia F.; Acquavia, Maria A.; Cataldi, Tommaso R. I.; Schmitt-Kopplin, Philippe; Buchicchio, Alessandro; Russo, Daniela; Milella, Luigi
- Abstract
Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of <italic>Fagioli di Sarconi</italic> beans (<italic>Phaseolus vulgaris</italic>, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (<italic>m/z</italic> 959.5213, [C48H79O19]+), Bb (<italic>m/z</italic> 943.5273, [C48H79O18]+), Bd (<italic>m/z</italic> 957.5122, [C48H77O19]+), and Be (<italic>m/z</italic> 941.5166, [C48H77O18]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (<italic>m/z</italic> 797.4680, [C42H69O14]+), αg (<italic>m/z</italic> 1085.5544, [C54H85O22]+), βg (<italic>m/z</italic> 1069.5600, [C54H85O21]+), and γg (<italic>m/z</italic> 923.5009, [C48H75O17]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR<bold>-</bold>MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with IC50 values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in <italic>Fagioli di Sarconi</italic> beans.The first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of <italic>Fagioli di Sarconi</italic> beans (<italic>P. vulgaris</italic> L.).<graphic></graphic>
- Subjects
TRITERPENOID saponins; LIQUID chromatography; HIGH performance liquid chromatography; CYCLOTRON resonance; ELECTROSPRAY ionization mass spectrometry
- Publication
Analytical & Bioanalytical Chemistry, 2018, Vol 410, Issue 5, p1561
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-017-0806-8