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- Title
Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants.
- Authors
Takane, Kenichi; Tanaka, Koji; Tajima, Shigeyuki; Okazaki, Katsuichiro; Kouchi, Hiroshi
- Abstract
A cDNA clone (URcot-35) was isolated from a soybean cotyledonary cDNA library using a cDNA clone (UR-nod-35) for nodule uricase II as a probe. URcot-35 was a 1,170-bp cDNA with an open reading frame that encoded a protein of putative 309 amino acids with a molecular mass of 35,137 Da. The nucleotide sequence of URcot-35 was identical to that of URnod-35.Expression of the URcot-35 gene in cotyledons was investigated by Northern dot-blot hybridization, by the reverse transcription-polymerase chain reaction with subsequent hybridization assays with the cDNA for nodule uricase II as a probe, and by immunoblotting analysis with a monoclonal antibody that was specific to nodule uricase II. The results suggested that the transcript of URcot-35 was present in developing cotyledons and that uricase II accumulated during the pod-filling stage. This is the first report of the isolation of cDNA for uricase II from non-symbiotic tissue and the results demonstrate that uricase II in soybean cotyledons is identical to that in soybean nodules.
- Subjects
GENE expression in plants; NODULINS; COTYLEDONS; SOYBEAN; ANTISENSE DNA; MOLECULAR cloning; PLANT gene isolation; REVERSE transcriptase polymerase chain reaction; NITROGEN fixation
- Publication
Plant & Cell Physiology, 1997, Vol 38, Issue 2, p149
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/oxfordjournals.pcp.a029145