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- Title
Membrane Thickness Changes Ion-Selectivity of Channel-Proteins.
- Authors
Garavaglia, MariaLisa; Dopinto, Silvia; Ritter, Markus; Fürst, Johannes; Saino, Stefano; Guizzardi, Fabiana; Jakab, Martin; Bazzini, Claudia; Vezzoli, Valeria; Dossena, Silvia; Rodighiero, Simona; Sironi, Chiara; Bottà, Guido; Meyer, Giuliano; Henderson, Robert M.; Paulmichl, Markus
- Abstract
The plasma membrane is a highly dynamic cell-barrier if the nature and distribution of its constituents are considered. Ion channels are embedded in these double lipid bilayers, which modulate their 3D-structures. The structure modulations by the lipid bilayer can assume such a degree that channel activation depends on them, as was shown for the KcsA potassium channel. Here we show that the cation-over-anion selectivity of reconstituted ICln channels can be varied by the thickness of a bilayer build of phosphatidylcholines. The shorter the acyl-chains and therefore the thinner the bilayers of the membrane are, the more potassium selective the channels are. In contrast, the longer the acyl-chains and therefore the thicker the membranes are, the more chloride selective the channels become.
- Subjects
CELL membranes; BIOLOGICAL membranes; BILAYER lipid membranes; LIPIDS; BIOMOLECULES
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2004, Vol 14, Issue 4-6, p231
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000080332