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- Title
Floral Initiation of Xanthium in Response to Application of an Extract from a Day-Neutral Plant.
- Authors
LINCOLN, RICHARD G.; MAYFIELD, DARWIN L.; HUTCHINS, ROBERT O.; CUNNINGHAM, ALAN; HAMNER, KARL C.; CARPENTER, BRUCE H.
- Abstract
A PROCEDURE for the preparation of a floral initiating extract from the leaf tissues of Xanthium strumarium has been described in an earlier report1. In general, the procedure involves the rapid freezing in liquid nitrogen of fresh leaf material obtained from flowering Xanthium plants. The frozen tissue is lyophilized at temperatures below 0° C. In recent work the continuous extraction procedure has been modified and extraction of the dry leaf material is now accomplished by several washings with cold (0-10° C.) absolute methanol. The methanol is removed at a low temperature by evaporation at reduced pressure in a Rinco apparatus. The residue is diluted with 1.5-5 times its weight of lanolin and the mixture applied as a coating to the under surface of the leaves of vegetative Xanthium test plants. All treated plants and their corresponding controls are maintained until dissection on strict long-day conditions. Three weeks after treatment the plants are dissected and the extent and degree of flowering is recorded according to a standard procedure2.
- Publication
Nature, 1962, Vol 195, Issue 4844, p918
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/195918a0