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- Title
Identification and manipulation of a novel locus to improve cell tolerance to short-chain alcohols in Escherichia coli.
- Authors
Chen, Yan; Wang, Ying; Chen, Tian-Hua; Yao, Ming-Dong; Xiao, Wen-Hai; Li, Bing-Zhi; Yuan, Ying-Jin
- Abstract
Escherichia coli KO11 is a popular ethanologenic strain, but is more sensitive to ethanol than other producers. Here, an ethanol-tolerant mutant EM was isolated from ultraviolet mutagenesis library of KO11. Comparative genomic analysis added by piecewise knockout strategy and complementation assay revealed EKO11_3023 (espA) within the 36.6-kb deletion from KO11 was the only locus responsible for ethanol sensitivity. Interestingly, when espA was deleted in strain W (the parent strain of KO11), ethanol tolerance was dramatically elevated to the level of espA-free hosts [e.g., MG1655 and BL21(DE3)]. And overexpression of espA in strains MG1655 and BL21(DE3) led to significantly enhanced ethanol sensitivity. In addition to ethanol, deletion of espA also improved cell tolerance to other short-chain (C2-C4) alcohols, including methanol, isopropanol, n-butanol, isobutanol and 2-butanol. Therefore, espA was responsible for short-chain alcohol sensitivity of W-strains compared to other cells, which provides a potential engineering target for alcohols production.
- Subjects
SYNTHETIC biology; LOCUS (Genetics); ESCHERICHIA coli; ISOPROPYL alcohol; BUTANOL; ULTRAVIOLET microscopy
- Publication
Journal of Industrial Microbiology & Biotechnology, 2018, Vol 45, Issue 7, p589
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-017-1996-y