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- Title
Extensive evaluation of a new LC–MS-MS method to quantify monofluoroacetate toxin in the kidney.
- Authors
Langston, James; Stump, Samuel; Filigenzi, Michael; Tkachenko, Andriy; Guag, Jake; Poppenga, Robert; Rumbeiha, Wilson K
- Abstract
Monofluoroacetate is a highly lethal toxin that causes death by inhibiting cellular adenosine triphosphate (ATP) production. The heart and brain are the primary target organs. Acute death is attributed to cardiac fibrillation and/or convulsions. Although it occurs naturally in some plants, a major source of animal intoxication is access to sodium monofluoroacetate (NaMFA) pesticide, which continues to be a concern in the USA and around the world despite restricted use in some countries including the USA. There are also concerns about misuse of this pesticide for malicious poisoning. Currently, a tissue-based diagnostic method for NaMFA intoxication in animals is lacking. There is a critical need by the veterinary diagnostic community for a simple, sensitive and reliable tissue-based diagnostic test to confirm NaMFA poisoning in animals. We have developed and extensively evaluated a sensitive novel liquid chromatography combined with tandem mass spectrometry method suitable for this purpose. The limits of detection and limits of quantitation are 1.7 and 5.0 ng/g, respectively. The accuracy and precision met or exceeded expectations. The method performance was verified using the incurred kidney obtained from animal diagnostic cases. This novel kidney-based method is now available for clinical use and can help with diagnostic purposes, including detecting potential issues related to animal foods.
- Subjects
LIQUID chromatography-mass spectrometry; TOXINS; PESTICIDES; ADENOSINE triphosphate; DETECTION limit
- Publication
Journal of Analytical Toxicology, 2024, Vol 48, Issue 4, p210
- ISSN
0146-4760
- Publication type
Article
- DOI
10.1093/jat/bkae032