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- Title
The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia.
- Authors
Küçük, Mutlu; Kaya, Mehmet; Orhan, Nurcan; Ekızoğlu, Oğuzhan; Arslan, Erol; Arican, Nadir; Gürses, Candan; Elmas, İmdat; Kalayci, Rivaze; Ahishali, Bülent
- Abstract
The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZinduced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
- Subjects
BLOOD-brain barrier; TETRAZOLES; SPASMS; PREECLAMPSIA; PATHOLOGICAL physiology; HYPERTENSION in pregnancy; AQUAPORINS; LABORATORY rats
- Publication
Turkish Journal of Veterinary & Animal Sciences, 2012, Vol 36, Issue 3, p311
- ISSN
1300-0128
- Publication type
Article
- DOI
10.3906/vet-1108-2