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- Title
DETECTION OF DNA AND ANTI-TOXOPLASMA GONDII ANTIBODIES IN ERRANT CATS (FELIS CATUS DOMESTICUS, LINNAEUS, 1758) CAPTURED BY THE ZOONOSES CONTROL CENTER OF GOIÂNIA, STATE OF GOIÁS, BRAZIL.
- Authors
ALVES REZENDE, HANSTTER HALLISON; SILVA LIMA, JAQUELINE ATAÍDE; ROBERTO GOMESJÚNIOR, ANTONIO; DE OLIVEIRA MELO, JADE; RIBEIRO STORCHIO, HELOÍSA; CRISTINA GOMES, TAYNARA; CLARE VINAUD, MARINA; MARIA DE CASTRO, ANA
- Abstract
The purpose of this study was to verify the seropositivity of IgG antiT. gondii antibodies in serum samples and to detect T. gondii DNA detection in tissue from stray cats captured by the Zoonosis Control Center (ZCC)of Goiânia, state of Goiás, during 2016. Antibodies were analyzed by means of an indirect hemagglutination assay (IHA), while DNA was detected by polymerase chain reaction (PCR), using primers that amplify the B1 gene in specimens of brain, muscle and heart from the animals. The serological analysis revealed that 87.4% (21/24) of the cats were positive and the molecular test indicated a positivity rate of 75% (18/24). These high rates of serological and molecular detection are worrisome for public health because they confirm the important role cats play in environmental contamination, and hence, in the transmission of toxoplasmosis to humans.
- Publication
Journal of Neotropical Biology / Revista de Biologia Neotropical, 2019, Vol 16, Issue 2, p84
- ISSN
1807-9652
- Publication type
Article