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- Title
Characterization of 'QTL-hotspot' introgression lines reveals physiological mechanisms and candidate genes associated with drought adaptation in chickpea.
- Authors
Barmukh, Rutwik; Roorkiwal, Manish; Dixit, Girish P; Bajaj, Prasad; Kholova, Jana; Smith, Millicent R; Chitikineni, Annapurna; Bharadwaj, Chellapilla; Sreeman, Sheshshayee M; Rathore, Abhishek; Tripathi, Shailesh; Yasin, Mohammad; Vijayakumar, Adiveppa G; Sagurthi, Someswar Rao; Siddique, Kadambot H M; Varshney, Rajeev K
- Abstract
' QTL-hotspot ' is a genomic region on linkage group 04 (CaLG04) in chickpea (Cicer arietinum) that harbours major-effect quantitative trait loci (QTLs) for multiple drought-adaptive traits, and it therefore represents a promising target for improving drought adaptation. To investigate the mechanisms underpinning the positive effects of ' QTL-hotspot ' on seed yield under drought, we introgressed this region from the ICC 4958 genotype into five elite chickpea cultivars. The resulting introgression lines (ILs) and their parents were evaluated in multi-location field trials and semi-controlled conditions. The results showed that the ' QTL-hotspot ' region improved seed yield under rainfed conditions by increasing seed weight, reducing the time to flowering, regulating traits related to canopy growth and early vigour, and enhancing transpiration efficiency. Whole-genome sequencing data analysis of the ILs and parents revealed four genes underlying the ' QTL-hotspot ' region associated with drought adaptation. We validated diagnostic KASP markers closely linked to these genes using the ILs and their parents for future deployment in chickpea breeding programs. The CaTIFY4b-H2 haplotype of a potential candidate gene CaTIFY4b was identified as the superior haplotype for 100-seed weight. The candidate genes and superior haplotypes identified in this study have the potential to serve as direct targets for genetic manipulation and selection for chickpea improvement.
- Subjects
PHYSIOLOGY; CHICKPEA; LOCUS (Genetics); SEED yield; DROUGHTS; GENES; NUCLEOTIDE sequencing
- Publication
Journal of Experimental Botany, 2022, Vol 73, Issue 22, p7255
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erac348