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- Title
Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper.
- Authors
Yanuhar, Uun; Musa, Muhammad; Arfiati, Diana; Sholihah, Qomariyatus; Khumaidi, Ach.; Caesar, Nico Rahman
- Abstract
Study aims to show the capability of Pigment-Protein Fractions extracted from C. vulgaris for treating the infection of Viral Nervous Necrosis (VNN) in Humpback grouper. Study was conducted by isolation and culture C. vulgaris after identified using 16 sRNA. PPF was extracted and analyzed using the SDS-page. Fish treatment were divided in four groups: (A) normal fish/control), (B) PPF administration into fish, (C) VNN induction into fish, and (D) both VNN and PPF induction into fish. β-Actin and MHC-1 response were evaluated using ImmunoRatio (IR) analysis. The results show that microalgae that local isolated from Indonesia is confirm as C. vulgaris with nucleotide sequences of 424 bp. Extracted PPF of C. vulgaris show a 33 kDa of protein molecule weight. Treatment in group B, C, and D able to increases the MHC-1 expression as much as 26.1%, 35%, and 66.9%, respectively. PCR quantification results in all PPF test treatments showed the presence of B-actin expression. Histopathology observation on eye tissue indicates occurred Necrosis (N) in group B, Vacuolation (V) and Necrosis (N) in group C. In group D, there are Vacuolation (V) and Necrosis (N) but less tissue damage compare than group B. It is indicates that C. vulgaris have a positive influence on the immune response of groupers (C. altivelis) after infected by VNN.
- Subjects
INDONESIA; CHLORELLA vulgaris; GROUPERS; VIRUS diseases; PROTEINS; PIGMENTS
- Publication
Systematic Reviews in Pharmacy, 2020, Vol 11, Issue 7, p352
- ISSN
0975-8453
- Publication type
Article