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- Title
Colony Growth of T Lymphocytes in vitro.
- Authors
Rozenszajn, L.A.; Goldman, I.; Zeevi, A.
- Abstract
Human lymphocytes stimulated with PHA in liquid phase (step 1) and then seeded in a two-layer soft agar system (step 2) grew and developed into T cell colonies. Colony formation was enhanced when the agar culture was supplemented with culture fluid obtained from phytohemagglutinin-treated lymphocytes (Ly-CF). Untreated lymphocytes plated directly in the soft agar system also developed into colonies if the culture medium contained Ly-CF. Mitogen-sensitized T lymphocytes produced a lymphocyte colony enhancing factor in the culture fluid which stimulated lymphocytes into colony formation. The best plating efficiency (1:250) was attained when blood mononuclear cells were seeded. When spleen cell culture fluid or a highly concentrated blood-adherent cell population was added to the soft agar, colony development was strongly inhibited. Monocytes-macrophages secrete a lymphocyte colony inhibiting factor in the culture medium. These lymphokines exert a competitive influence on T cells and thus control and regulate clonal proliferation. Copyright © 1979 S. Karger AG, Basel
- Publication
Acta Haematologica, 1979, Vol 62, Issue 5/6, p315
- ISSN
0001-5792
- Publication type
Article
- DOI
10.1159/000207595