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- Title
Inhibition of matrix metalloproteinases and toxicity of gold and platinum nanoparticles in L929 fibroblast cells.
- Authors
Hashimoto, Masanori; Yamaguchi, Satoshi; Sasaki, Jun‐Ichi; Kawai, Koji; Kawakami, Hayato; Iwasaki, Yasuhiko; Imazato, Satoshi
- Abstract
This study evaluated the inhibition of matrix metalloproteases ( MMPs) and cellular responses elicited by gold (Au) and platinum (Pt) nanoparticles ( NPs). The interaction of MMP-1 and NPs was evaluated using an MMP assay kit. The cultured L929 cells were exposed to various concentrations of NPs. The cellular responses to NPs were examined using a cytotoxicity assay (that evaluated cell viability and lactic dehydrogenase production), real-time polymerase chain reaction (RT-qPCR), and transmission electron microscopy. Both types of NPs, when used at concentrations above 10 μg ml−1, inhibited MMP-1 activity. No cytotoxic effects were found when the cells were exposed to Au NPs. In contrast, Pt NPs, at both 100 and 400 μg ml−1, induced cytotoxicity. No inflammatory responses (production of interleukin-6 and tumor necrosis factor-alpha) to NPs were identified by RT-qPCR. The negative surface charge of NPs ( COOH−) binds to the Zn2+ of the MMP active center by chelation, leading to MMP inhibition. Gold nanoparticles are plausible candidates for MMP inhibitors in resin-bonding materials because they effectively inhibit MMP-1 activity without cytotoxic or inflammatory effects.
- Subjects
FIBROBLASTS; ENZYME metabolism; ELECTRON microscopy; GOLD; LACTATE dehydrogenase; RESEARCH methodology; NANOPARTICLES; PLATINUM; POLYMERASE chain reaction; RESEARCH funding; STATISTICS; TISSUE culture; DATA analysis; IN vitro studies; ONE-way analysis of variance; PHYSIOLOGY
- Publication
European Journal of Oral Sciences, 2016, Vol 124, Issue 1, p68
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1111/eos.12235