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- Title
NK22 Cells in the Uterine Mid-Secretory Endometrium and Peripheral Blood of Women with Recurrent Pregnancy Loss and Unexplained Infertility.
- Authors
Kamoi, Mai; Fukui, Atsushi; Kwak ‐ Kim, Joanne; Fuchinoue, Kohei; Funamizu, Ayano; Chiba, Hitomi; Yokota, Megumi; Fukuhara, Rie; Mizunuma, Hideki
- Abstract
Problem We aimed to investigate natural killer 22 ( NK22) cells in the peripheral blood and the uterine endometrium of women with unexplained recurrent pregnancy loss ( URPL) and unexplained infertility ( UI). Method of study Peripheral blood and endometrial samples were collected from women with URPL ( n = 43) and UI ( n = 38). Intracellular cytokine production, such as IL-22, IFN-γ and TNF-α, and the expression of NKp46 on NK cells were analyzed by three-color flow cytometry. Results The percentages of endometrial CD56+/ IL-22+ and CD56dim/ IL-22+ cells in women with URPL were significantly higher than those of UI ( P < 0.05, respectively). In addition, the percentage of CD56bright/ IL-22+ cells in women with RPL was negatively correlated with those of CD56bright/ IFN-γ+ and CD56bright/ TNF-α+ in both peripheral blood and endometrial NK cells. This was not seen in women with UI. The percentage of CD56bright/ IL-22+ cells was negatively correlated with CD56bright/ NKp46 expressing NK cells in peripheral blood. Conclusion Endometrial NK22 cells are differently regulated in women with URPL and UI. Women with URPL have higher level of NK22 cells with a potential to induce NK2 shift than women with UI.
- Subjects
KILLER cells; INFERTILITY; FLOW cytometry; TUMOR necrosis factors; ENDOMETRIUM
- Publication
American Journal of Reproductive Immunology, 2015, Vol 73, Issue 6, p557
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12356