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- Title
Expression and distribution of metabotropic GABA receptor subtypes GABA<sub>B</sub> R1 and GABA<sub>B</sub> R2 during rat neocortical development.
- Authors
López‐Bendito, G.; Shigemoto, R.; Kulik, A.; Paulsen, O.; Fairén, A.; Luján, R.
- Abstract
Abstract To understand the possible contribution of metabotropic γ-aminobutyric acid receptors (GABAB R) in cortical development, we investigated the expression pattern and the cellular and subcellular localization of the GABAB R1 and GABAB R2 subtypes in the rat neocortex from embryonic day 14 (E14) to adulthood. At the light microscopic level, both GABAB R1 and GABAB R2 were detected as early as E14. During prenatal development, both subtypes were expressed highly in the cortical plate. Using double immunofluorescence, GABAB R1 colocalized with GABAB R2 in neurons of the marginal zone and subplate, indicating that these proteins are coexpressed and could be forming functional GABAB Rs during prenatal development in vivo . In contrast, only GABAB R1 but not GABAB R2 was detected in the tangentially migratory cells in the lower intermediate zone. During postnatal development, immunoreactivity for GABAB R1 and GABAB R2 was distributed mainly in pyramidal cells. Discrete GABAB R1-immunopositive cell bodies of interneurons were present throughout the neocortex. In addition, GABAB R1 but not GABAB R2 was found in identified Cajal-Retzius cells in layer I. At the electron microscopic level, immunoreactivity for GABAB R1 and GABAB R2 was found in dendritic spines and dendritic shafts at extrasynaptic and perisynaptic sites throughout postnatal development. We further demonstrated the presynaptic localization of GABAB R1 and GABAB R2, as well as the association of the receptors with asymmetrical synaptic junctions. These results indicate potentially important roles for the GABAB Rs in the regulation of migratory processes during corticogenesis and in the modulation of synaptic transmission during early development of cortical circuitry.
- Subjects
GABA; CEREBRAL cortex; NEURAL transmission
- Publication
European Journal of Neuroscience, 2002, Vol 15, Issue 11, p1766
- ISSN
0953-816X
- Publication type
Article
- DOI
10.1046/j.1460-9568.2002.02032.x