We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Single-Cell RNA Sequencing Resolves Molecular Relationships Among Individual Plant Cells.
- Authors
Kook Hui Ryu; Ling Huang; Hyun Min Kang; Schiefelbein, John
- Abstract
Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes from protoplasts of more than 10,000 Arabidopsis (Arabidopsis thaliana) root cells. We find that all major tissues and developmental stages are represented in this single-cell transcriptome population. Further, distinct subpopulations and rare cell types, including putative quiescent center cells, were identified. A focused analysis of root epidermal cell transcriptomes defined developmental trajectories for individual cells progressing from meristematic through mature stages of root-hair and nonhair cell differentiation. In addition, single-cell transcriptomes were obtained from root epidermis mutants, enabling a comparative analysis of gene expression at single-cell resolution and providing an unprecedented view of the impact of the mutated genes. Overall, this study demonstrates the feasibility and utility of scRNA-seq in plants and provides a first-generation gene expression map of the Arabidopsis root at single-cell resolution.
- Publication
Plant Physiology, 2019, Vol 179, Issue 4, p1444
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1104/pp.18.01482