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- Title
Analysis of Trypanosoma cruzi metacyclogenesis in different Discrete Typing Units (DTUs).
- Authors
Cáceres Bernal, Tatiana Marcela; Cruz Saavedra, Lissa; David Ramirez, Juan
- Abstract
Trypanosoma cruzi has a wide genetic variability which has favored the division of this species into subgroups that can be identified by common molecular, genetic, biochemical and immunological markers. Currently, it is classified into six discrete typing units of TcI-TcVI and TcBat. These have differential characteristics related to their prevalence, geographic distribution, hosts, and vectors. Also, biological characteristics such as virulence and replication rates. Metacyclogenesis is a very important process in the life cycle of T. cruzi since, at this stage, non-infectious epimastigotes (EP) become infectious metacyclic trypomastigotes (MT). Therefore, the aim of this study was to determine whether differences in the time required to reach the peak of metacyclogenesis in cultures of different DTUs. T. cruzi strains Tel (DA/MG), Tell (Y), TcIII (845), TcIV (85) and TcVI (Tulahuen) were cultured with a concentration of lxlO8 EP/mL and three biological replicates in liver infusion medium (LIT) supplemented with 1C% fetal bovine serum at 26 °C, conducive to nutritional stress conditions that give rise to the process of metacyclogenesis. They were quantified by Neubauer chamber for 12 days and by Giemsa staining where 300 parasite morphologies were classified into EP and MT. Results were tabulated in Excel and analysis of variance was performed by R software to determine statistical significance. The starting point of metacyclogenesis for DA was found at day 3, MG (4), Tell (6), TcIII and TcIV (3), and TcVI (4). For strains belonging to the DTU Tel only statistically significant differences were found from day 5. However, the number of MT was higher in MG than in DA (52'000.000 MT/9'000.000 MT). On the other hand, the strain that presented a lower number of MT was TcIV. The data presented a normal distribution and in the two-way ANOVA analysis statistically significant differences were found with a P value <0.0001 for the number of metacyclic trypomastigotes as a function of strain/day. The T. cruzi strain that presented a higher efficiency in the metacyclogenesis process was the MG strain, which shows that there are differences not only between DTUs but additionally between strains of the same DTUs, which could be related to circulation and transmission of the same.
- Subjects
TRYPANOSOMA cruzi; LIFE cycles (Biology); TWO-way analysis of variance; BIOMARKERS; GAUSSIAN distribution; GENETIC variation; SOMATIC embryogenesis
- Publication
Actualidades Biológicas, 2022, Vol 44, p37
- ISSN
0304-3584
- Publication type
Article