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- Title
A micromethod to measure ammonia.
- Authors
Cheema-Dhadli, Surinder; Hamat, Richard; Sonnenberg, Harrald; Halperin, Mitchell
- Abstract
We have described a simple technique for the measurement of picomoles or nanomoles of ammonium. Samples can be easily processed on the day of an experiment. Analysis of 14C-glutamate can be done at a later date by an accurate sensitive and reliable method. We can readily process 50 samples per day. The sensitivity can be increased by using smaller volumes of ammonia-free solutions, pre-reacting endogenous ammonia with nonradioactive 2-oxoglutarate, and increasing the specific activity of 2-oxoglutarate. The volumes must, however, be very low so as not to diminish the ammonium concentration and thereby limit glutamate formation.
- Subjects
KIDNEY tubules; PHYSIOLOGY; AMMONIA; NITROGEN compounds; GLUTAMATE dehydrogenase; GLUTAMIC acid; RADIOCHEMICAL analysis
- Publication
Kidney International, 1981, Vol 19, Issue 1, p80
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1981.10