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- Title
From single DNA adducts measurement to DNA adductomics in molecular epidemiology of cancer.
- Authors
Baer-Dubowska, Wanda; Szaefer, Hanna
- Abstract
Environmental carcinogens exert their carcinogenic effects by forming DNA adducts. This type of DNA damage can also be formed endogenously as a result of, e.g., oxidative damage. Unrepaired DNA adducts may induce mutations in critical genes, leading to the initiation of chemical carcinogenesis. Therefore, detection, identification, and quantification of DNA adducts is essential for cancer risk assessment. Over the last 50 years, the major DNA adducts formed by different classes of environmental carcinogens were characterized. With the development of techniques such as 32P-postlabeling, their measurement was implemented into molecular epidemiology. Advances in liquid chromatography-tandem mass spectrometry (LC-MS) made the measurement of adducts more precise and allowed to gain knowledge about their identity and structures. Therefore, these new techniques opened the way to DNA adductomics, the "omics" approach investigating DNA adducts comprehensively, similarly to proteomics. This review presents the historical perspective of DNA adducts research and the emerging field of adductomics.
- Subjects
DNA adducts; EPIDEMIOLOGY of cancer; MOLECULAR epidemiology; LIQUID chromatography-mass spectrometry; CHEMICAL carcinogenesis
- Publication
Advances in Biochemistry / Postepy Biochemii, 2024, Vol 70, Issue 1, p52
- ISSN
0032-5422
- Publication type
Article
- DOI
10.18388/pb.2021_509