We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polyphenol Profile, Antioxidant and Hypoglycemic Activity of Acalypha hispida Leaf Extract.
- Authors
ALFARISI, H.; SA'DIAH, S.; WRESDIYATI, T.
- Abstract
This investigation is aimed at evaluating the polyphenol profile, antioxidant, a-glucosidase and a-amylase inhibitory and the hypoglycemic activities of Acalypha hispida leaf extract in vivo. Powdered Acalypha hispida leaves were macerated with 70 and 96% ethanol and decocted in distilled water. The filtrates were evaporated to obtain dry extracts. The extracts were found to have a polyphenol profile (ultraperformance liquid chromatography quadrupole time-of-flight) and to exhibit antioxidant activity (2,2-diphenyl-1-picrylhydrazyl) and α-glucosidase and a-amylase inhibition (spectrophotometer) in vitro and a hypoglycemic effect in vivo. This study revealed that the number of polyphenol subclasses of the 96% ethanol extract was greater than that of the other extracts. The 70 and 96% ethanol and aqueous extracts exhibited strong antioxidant activity (IC50<10 µg/ml). The 96% ethanol extract showed very high inhibition of α-glucosidase and α-amylase activities. A hypoglycemic assay of the 96% ethanol extract at 300 mg/kg dose exhibited a significant hypoglycemic activity in experimental rats. The present results suggested that the ethanol extract of Acalpha hispida leaves could be an effective hyperglycemic agent acting through inhibiting α-glucosidase and α-amylase.
- Subjects
ANTIOXIDANTS; EXTRACTS; DISTILLED water; LIQUID chromatography; GLUCOSE tolerance tests
- Publication
Indian Journal of Pharmaceutical Sciences, 2020, Vol 82, Issue 2, p291
- ISSN
0250-474X
- Publication type
Article
- DOI
10.36468/pharmaceutical-sciences.649