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- Title
Identification of Maillard reaction products on peanut allergens that influence binding to the receptor for advanced glycation end products.
- Authors
Mueller, G. A.; Maleki, S. J.; Johnson, K.; Hurlburt, B. K.; Cheng, H.; Ruan, S.; Nesbit, J. B.; Pomés, A.; Edwards, L. L.; Schorzman, A.; Deterding, L. J.; Park, H.; Tomer, K. B.; London, R. E.; Williams, J. G.
- Abstract
Background Recent immunological data demonstrated that dendritic cells preferentially recognize advanced glycation end product ( AGE)-modified proteins, upregulate expression of the receptor for AGE ( RAGE), and consequently bias the immune response toward allergy. Methods Peanut extract was characterized by mass spectrometry ( MS) to elucidate the specific residues and specific AGE modifications found in raw and roasted peanuts and on rAra h 1 that was artificially glycated by incubation with glucose or xylose. The binding of the RAGE-V1C1 domain to peanut allergens was assessed by PAGE and Western analysis with anti-Ara h 1, 2, and 3 antibodies. IgE binding to rAra h 1 was also assessed using the same methods. Results AGE modifications were found on Ara h 1 and Ara h 3 in both raw and roasted peanut extract. No AGE modifications were found on Ara h 2. Mass spectrometry and Western blot analysis demonstrated that RAGE binds selectively to Ara h 1 and Ara h 3 derived from peanut extract, whereas the analysis failed to demonstrate Ara h 2 binding to RAGE. rAra h 1 with no AGE modifications did not bind RAGE; however, after AGE modification with xylose, rAra h 1 bound to RAGE. Conclusions AGE modifications to Ara h 1 and Ara h 3 can be found in both raw and roasted peanuts. Receptor for AGE was demonstrated to selectively interact with AGE-modified rAra h 1. If sensitization to peanut allergens occurs in dendritic cells via RAGE interactions, these cells are likely interacting with modified Ara h 1 and Ara h 3, but not Ara h 2.
- Subjects
MAILLARD reaction; PEANUT allergy; DENDRITIC cells; IMMUNE response; MASS spectrometry; WESTERN immunoblotting
- Publication
Allergy, 2013, Vol 68, Issue 12, p1546
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/all.12261