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- Title
KGF Enhances Oral Epithelial Adhesion and Rete Peg Elongation via Integrins.
- Authors
Sa, G. L.; Xiong, X. P.; Ren, J. G.; Wang, J. Y.; Xia, H. F.; Liu, Z. K.; He, S. G.; Zhao, Y. F.
- Abstract
Oral epithelial adhesion to the lamina propria underlies the physiologic function of the oral mucosa and contributes to resisting bacterial invasion, preventing body fluid loss, and maintaining routine chewing; thus, understanding the factors that positively influence oral epithelial adhesion is a research topic of great interest. Rete pegs contribute to oral epithelial adhesion by enlarging the contact areas, whereas integrins are the major molecules that mediate epithelial cell adhesion to the basement membrane. Keratinocyte growth factor (KGF) can promote both rete peg elongation in the skin and the expression of integrins in various cell types. Herein, we tested the effects of submucosal injection of KGF in the ventral surfaces of rat tongues on oral epithelial adhesion. The data confirmed that topical injection of KGF elevated the adhesive forces, elongated the rete pegs, and increased the abundance of integrins, KGF, and KGF receptor on the rat tongue ventral surface. However, HYD-1 (Lys-Ile-Lys-Met-Val-Ile-Ser-Trp-Lys-Gly), an integrin antagonist, inhibited the KGF-enhanced epithelial adhesion and rete peg elongation. Moreover, KGF promoted the expression of integrin subunits α6, β4, α3, and β1 in human immortalized oral epithelial cells in 2- and 3-dimensional culture systems. In vitro cell attachment assays demonstrated that HYD-1 inhibited the adhesion of human immortalized oral epithelial cells on Matrigel. Strikingly, the expression of integrins, KGF, and KGFR in human masticatory mucosae with longer rete pegs was more abundant than that in the lining mucosae with shorter rete pegs. In addition, rete peg lengths were positively correlated with the expression levels of integrins, KGF, and KGF receptor. These findings indicate that KGF strengthens oral epithelial adhesion and rete peg elongation via integrins.
- Subjects
KERATINOCYTE growth factors; EPITHELIUM; CELL-matrix adhesions; INTEGRIN genetics; ORAL mucosa; REGULATION of body fluids; GENE expression; FIBROBLAST growth factors; PHYSIOLOGY; ANIMAL experimentation; ANIMALS; CELL culture; CELL physiology; CELL receptors; EPITHELIAL cells; GROWTH factors; OLIGOPEPTIDES; POLYMERASE chain reaction; RATS; WESTERN immunoblotting
- Publication
Journal of Dental Research, 2017, Vol 96, Issue 13, p1546
- ISSN
0022-0345
- Publication type
journal article
- DOI
10.1177/0022034517720360