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- Title
Effect of Doxorubicin on Myocardial Concentration of Lycopene and its Metabolism in Wistar rats.
- Authors
Anjos Ferreira, Ana Lucia; Kyung-Jin Yeum; Matsubara, Luiz Shiguero; Matsubara, Beatriz Bojikian; Nascimento, Maria Carolina M. O.; Russell, Robert Mitchell; Krinsky, Norman I.; Tang, Guangwen
- Abstract
The mechanism of doxorubicin-induced cardiotoxicity may be related to free radical production. Wistar rats (n=96) were randomly assigned to either a control (C), Lycopene (L), Doxorubicin (D) or Doxorubicin + Lycopene (DL) group. The L and DL groups received lycopene (5 mg/Kg body wt/d by gavage) for 7 wks. The D and DL groups received 4 mg doxorubicin/Kg body wt/d intraperitoneally at the 3rd, 4th, 5th & 6th wks and were killed at the 7th wk for analyses. Myocardial tissue lycopene levels and total antioxidant performance (TAP) were analyzed by HPLC and fluorometry, respectively. Lycopene metabolism was determined by incubating lycopene with intestinal mucosa and lipoxygenase (LOX) and analyzed with HPLC & APCI mass spectroscopy. Myocardial tissue lycopene levels in DL & L were similar. TAP values adjusted for tissue protein were higher in myocardium of D than those of C (p=0.002). Oxidative cleavage of lycopene was significantly lower in D as compared to those of C when lycopene was incubated with intestinal mucosa with LOX. Our results showed that lycopene was not depleted in myocardium of lycopene-supplemented rats treated with doxorubicin and that higher antioxidant capacity in myocardium and less oxidative cleavage of lycopene in intestinal mucosa of doxorubicin treated rats suggests an antioxidant role of doxorubicin rather than prooxidant.
- Subjects
DOXORUBICIN; LYCOPENE; METABOLISM; RATS; MYOCARDIUM
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA350
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.5.a350