We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes.
- Authors
Hyejin Lee; Hua Li; Minsoo Noh; Jae-Ha Ryu
- Abstract
The fruit of Psoralea corylifolia L. (Fabaceae) (PC), known as "Bo-Gol-Zhee" in Korea has been used as traditional medicine. Ethanol and aqueous extracts of PC have an anti-hyperglycemic effect by increasing plasma insulin levels and decreasing blood glucose and total plasma cholesterol levels in type 2 diabetic rats. In this study, we purified six compounds from PC and investigated their anti-diabetic effect. Among the purified compounds, bavachin most potently accumulated lipids during adipocyte differentiation. Intracellular lipid accumulation was measured by Oil Red-O (ORO) cell staining to investigate the effect of compounds on adipogenesis. Consistently, bavachin activated gene expression of adipogenic transcriptional factors, proliferator-activated receptor (PPAR) and CCAAT/enhancer binding protein- (C/EBP). Bavachin also increased adiponectin expression and secretion in adipocytes. Moreover, bavachin increased insulin-induced glucose uptake by differentiated adipocytes and myoblasts. In differentiated adipocytes, we found that bavachin enhanced glucose uptake via glucose transporter 4 (GLUT4) translocation by activating the Akt and 51AMP-activated protein kinase (AMPK) pathway in the presence or absence of insulin. These results suggest that bavachin from Psoralea corylifolia might have therapeutic potential for type 2 diabetes by activating insulin signaling pathways.
- Subjects
ADIPONECTIN; PEROXISOME proliferator-activated receptors; DIAGNOSIS of diabetes; ADIPOGENESIS; PSORALEA; FAT cells; GENE expression; CELLULAR signal transduction
- Publication
International Journal of Molecular Sciences, 2016, Vol 17, Issue 4, p527
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms17040527