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- Title
Accurate determination of microbial diversity from 454 pyrosequencing data.
- Authors
Quince, Christopher; Lanzén, Anders; Curtis, Thomas P.; Davenport, Russell J; Hall, Neil; Head, Ian M; Read, L Fiona; Sloan, William T.
- Abstract
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.
- Subjects
ALGORITHMS; RECOMBINANT DNA; BIOLOGICAL classification; PHYSIOLOGICAL effects of noise; MICROBIAL diversity
- Publication
Nature Methods, 2009, Vol 6, Issue 9, p639
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1361