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- Title
A cytotoxic peptide from a marine sponge exhibits ion channel activity through vectorial-insertion into the membrane
- Authors
Iwamoto, Masayuki; Shimizu, Hirofumi; Muramatsu, Ikunobu; Oiki, Shigetoshi
- Abstract
Abstract: A cytotoxic peptide, polytheonamide B (pTB), from marine sponge was examined for cytotoxic spectrum and specific activity to mammalian cells was demonstrated. pTB is composed of alternative D- and L-amino acid residues throughout the 48-mer peptide. This suggests the formation of a β-helix similar to gramicidin channels. Planar bilayer experiments revealed that pTB forms monovalent cation-selective channels, being compatible with the inner pore diameter of ∼4Å for a β-helical structure. pTB penetrated vectorially into the membrane, formed a channel by means of a single molecule, and remained in the membrane. These functional properties may account for specific cytotoxic activity.
- Subjects
PEPTIDES; SPONGES (Invertebrates); AMIDES; CELL membranes; AMINO acids; CATIONS
- Publication
FEBS Letters, 2010, Vol 584, Issue 18, p3995
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.08.007