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- Title
Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells
- Authors
Masumi, Atsuko; Hamaguchi, Isao; Kuramitsu, Madoka; Mizukami, Takuo; Takizawa, Kazuya; Momose, Haruka; Naito, Seishiro; Yamaguchi, Kazunari
- Abstract
Abstract: Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-γ treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.
- Subjects
INTERFERONS; HEMATOPOIETIC stem cells; FLUOROURACIL; GENE expression; INFLAMMATION; GENETIC regulation; GENETICS
- Publication
FEBS Letters, 2009, Vol 583, Issue 21, p3493
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.10.006