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- Title
Importance of C-terminal flexible region of 4E-binding protein in binding with eukaryotic initiation factor 4E
- Authors
Mizuno, Atsuo; In, Yasuko; Fujita, Yuki; Abiko, Fumi; Miyagawa, Hiroo; Kitamura, Kunihiro; Tomoo, Koji; Ishida, Toshimasa
- Abstract
Abstract: Although the α-helical Y(X)4Lϕ containing region of eIF4E-binding protein (4EBP) is the major binding region with eukaryotic initiation factor 4E (eIF4E), the roles of its N- and C-terminal regions in the binding are hardly known. To clarify the roles of these flexible regions in the interaction, the binding features of the sequentially N-, C-, or both-terminal-residue-deleted 4EBP2 mutants were investigated by surface plasmon resonance (SPR) analysis. It was shown that the C-terminal His74-Glu89 sequence has an auxiliary, but indispensable, function in stabilizing the binding to eIF4E. The possible interaction with eIF4E was estimated by molecular dynamics simulation. This is the first report on the importance of the C-terminal flexible region in the eIF4E-binding regulation of 4EBP.
- Subjects
CARRIER proteins; EUKARYOTIC cells; SURFACE plasmon resonance; MOLECULAR dynamics
- Publication
FEBS Letters, 2008, Vol 582, Issue 23/24, p3439
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.09.003