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- Title
TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei
- Authors
Hong, Yeonchul; Nagamune, Kisaburo; Ohishi, Kazuhito; Morita, Yasu S.; Ashida, Hisashi; Maeda, Yusuke; Kinoshita, Taroh
- Abstract
Abstract: Glycosylphosphatidylinositol (GPI) is widely used by eukaryotic cell surface proteins for membrane attachment. De novo synthesized GPI precursors are attached to proteins post-translationally by the enzyme complex, GPI transamidase. TbGPI16, a component of the trypanosome transamidase, shares similarity with human PIG-T. Here, we show that TbGPI16 is the orthologue of PIG-T and an essential component of GPI transamidase by creating a TbGPI16 knockout. TbGPI16 forms a disulfide-linked complex with TbGPI8. A cysteine to serine mutant of TbGPI16 was unable to fully restore the surface expression of GPI-anchored proteins upon transfection into the knockout cells, indicating that its disulfide linkage with TbGPI8 is important for the full transamidase activity.
- Subjects
PROTEINS; EUKARYOTIC cells; TRYPANOSOMA brucei; BIOCHEMISTRY
- Publication
FEBS Letters, 2006, Vol 580, Issue 2, p603
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.12.075