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- Title
Biosynthesis of Benzoylformic Acid from Benzoyl Cyanide with a New Bacterial Isolate of Brevibacterium sp. CCZU12-1.
- Authors
He, Yu-Cai; Pan, Xue-He; Xu, Xiao-Feng; Wang, Li-Qun
- Abstract
Brevibacterium sp. CCZU12-1 with high nitrilase activity could effectively hydrolyze benzoyl cyanide into benzoylformic acid. After the culture optimization, the preferred carbon sources, nitrogen sources, and inducer were glucose (10 g/L), a composite of peptone (10 g/L) plus yeast extract (2.5 g/L), and ε-caprolactam (2.0 mM), respectively. After the reaction optimization, the optimum reaction temperature, reaction pH, organic cosolvent, and metal ion were 30 °C, 7.0, ethanol (2 %, v/ v), and Ca (0.1 mM), respectively. At biotransformation of 120-mM benzoyl cyanide for 24 h, the yield of benzoylformic acid reached 91.8 %. Moreover, the microbial nitrilase from Brevibacterium sp. CCZU12-1 could hydrolyze various nitriles, and it significantly exhibited high nitrilase activity against benzoyl cyanide, 3-cyanopyridine, and α-cyclohexyl-mandelonitrile.
- Subjects
ORGANIC solvent analysis; BREVIBACTERIUM; BIOTRANSFORMATION in microorganisms; ORGANIC solid state chemistry; BIOCHEMICAL engineering; LEAVENING agents
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 6, p3223
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-014-0764-y