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- Title
Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs.
- Authors
Maharani, Dyah; Park, Hee-Bok; Lee, Jae-Bong; Yoo, Chae-Kyoung; Lim, Hyun-Tae; Han, Sang-Hyun; Lee, Sung-Soo; Ko, Moon-Suck; Cho, In-Cheol; Lee, Jun-Heon
- Abstract
Fatty acid (FA) composition is one of the most important parameters for the evaluation of meat quality. The stearoyl-CoA desaturase (SCD) gene is considered a positional candidate gene affecting FA composition in SSC14, based on previous quantitative trait loci studies. To evaluate the association of the SCD gene with FA composition in a Korean native pig × Landrace F intercross population, we genotyped six single nucleotide polymorphisms (SNPs) of the SCD gene located in promoter region [2 SNPs (g.−353T>C, g.−233T>C)], exonic region [1 SNP (g.817C>T) in exon 2] and 3′ UTR [3 SNPs (g.13311C>G, g.14384G>A, and g.14424C>T)] identified by massively parallel sequencing technology. Eighteen FA composition traits were measured in more than the 950 F animals. A mixed-effect model was used to evaluate associations between these SNPs and FA composition traits in the F intercross population. A detailed investigation detected that the five FA composition traits [palmitoleic acid (C16:1), stearic acid (C18:0), arachidic acid (C20:0), saturated FA, and unsaturated FA] were highly significant ( P < 4.7 × 10; C20:0) in association with the SNP g.−233T>C, SNP g.817C>T, SNP g.13311C>G and SNP g.14384G>A in the SCD gene, whereas SNP g.14424C>T was only significantly associated with palmitoleic acid (C16:1, P = 1.4 × 10). No significant association of FA composition traits with SNP g.−353T>C was detected. In particular, the SNP g.14384G>A accounted for 30.6 % of the additive genetic variance of palmitoleic acid ( P = 1.9 × 10). These results suggest the SCD gene has a strong effect on FA composition in the crossbred pig population.
- Subjects
GENETIC code; LANDRACE swine; SWINE physiology; FATTY acids; SINGLE nucleotide polymorphisms
- Publication
Molecular Biology Reports, 2013, Vol 40, Issue 1, p73
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-012-2014-0