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- Title
Targeted gene delivery to differentiated skeletal muscle: A tool to study dedifferentiation.
- Authors
Jamie I. Morrison; Sara Lööf; Pingping He; Peter Aleström; Philippe Collas; András Simon
- Abstract
Cellular dedifferentiation is required for functional regeneration in salamanders. Dedifferentiating multinucleate skeletal muscle gives rise to mononucleate cells during limb regeneration. Efficient methods and tools must be developed in order to understand the molecular cues underlying dedifferentiation. Here we describe a non‐viral method to express extra‐chromosomal DNA exclusively in terminally differentiated muscle without the need for cell purification steps. After cytoplasmic injection of various expression vectors into myotubes or myofibres, we detect long‐lasting mRNA and protein expression in up to 70% of the injected cells. The combination of the transfection protocol with live imaging allows a time‐ and cost‐effective screen of candidate genes in terminally differentiated muscle cells of both amphibian and mammalian origin. Developmental Dynamics 236:481–488, 2007. © 2006 Wiley‐Liss, Inc.
- Subjects
CELL differentiation; SMOOTH muscle regeneration; GENE expression; MUSCLE cells
- Publication
Developmental Dynamics, 2007, Vol 236, Issue 2, p481
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.21019