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- Title
Analysis of differentially expressed proteins in late-stationary growth phase of Mycobacterium tuberculosis H37 Rv.
- Authors
Ang, Kai‐Cheen; Ibrahim, Pazilah; Gam, Lay‐Harn
- Abstract
Mycobacterium tuberculosis is a causative agent of tuberculosis ( TB). The ability of M. tuberculosis to be quiescent in the cell has caused the emergence of latent infection. A comprehensive proteomic analysis of M. tuberculosis H37 Rv over three growth phases, namely mid-log (14-day culture), early stationary (28-day culture), and late stationary (50-day culture), was performed in order to study the change in proteome from the mid-log phase to late-stationary phase. Combination methods of two-dimensional electrophoresis (2- DE) and tandem mass spectrometry were used to generate proteome maps of M. tuberculosis at different growth phases. Ten proteins were detected differentially expressed in the late-stationary phase compared with the other two phases. These proteins were SucD, TrpD, and Rv2161c, which belong to metabolic pathway proteins; FadE5, AccD5, DesA1, and Rv1139c are proteins involved in cell wall or lipid biosynthesis, whereas TB21.7 and Rv3224 are conserved hypothetical proteins with unknown function. A surface antigen protein, DesA1, was not detectable in the late-stationary phase, although present in both log and early-stationary phases. The changes in the expression levels of these proteins were in line with the growth environment changes of the bacteria from mid-log phase to late-stationary phase. The information gathered may be valuable in the intervention against latent TB infection.
- Subjects
MYCOBACTERIUM tuberculosis; PROTEIN analysis; TWO-dimensional electrophoresis; GROWTH curves (Statistics); TANDEM mass spectrometry techniques; CELL surface antigens; BIOMARKERS
- Publication
Biotechnology & Applied Biochemistry, 2014, Vol 61, Issue 2, p153
- ISSN
0885-4513
- Publication type
Article
- DOI
10.1002/bab.1137