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- Title
Topical Application of Peptide Nucleic Acid Antisense Oligonucleotide for MMP-1 and Its Potential Anti-Aging Properties.
- Authors
Lee, Young In; Lee, Sang Gyu; Jung, Inhee; Suk, Jangmi; Baeg, Chaemin; Han, Seon-Young; Seo, Jeong Yeon; Jung, Daram; Jeon, Yeasel; Lee, Ju Hee
- Abstract
Matrix metalloproteinase-1 (MMP-1) is a zinc-containing endopeptidase that degrades dermal collagen and other extracellular matrix molecules. It is recognized as one of the most important indicators of cellular senescence and age-related skin changes. Here, we introduced a novel MMP-1 peptide nucleic acid (PNA) derivative—PNA-20 carboxyethyl fluorene (CEF)—which can interact with and consequently silence the MMP-1 gene sequence. The investigation on the efficacy of PNA-20 CEF in MMP-1 silencing in human dermal fibroblasts revealed significantly decreased expression of MMP-1 at both gene and protein levels. Treatment with PNA-20 CEF showed significantly increased expression of collagen I protein, indicating its potential role in preventing the degradation of collagen I and consequently combating the skin aging process. Its topical application on 3D human skin tissue showed successful absorption into the epidermis and the upper dermis. Furthermore, the additional 4-week single-arm prospective study on 21 Asian women revealed improvements in facial wrinkles, skin moisture, elasticity, and density after the use of the topical PNA-20 CEF cosmeceutical formulation. Additional in-vitro and ex-vivo studies are needed for a comprehensive understanding of the skin anti-aging effects of MMP-1 PNA.
- Subjects
PEPTIDE nucleic acids; ANTISENSE nucleic acids; TOPICAL drug administration; MATRIX metalloproteinases; WRINKLES (Skin); SKIN aging; AGING prevention
- Publication
Journal of Clinical Medicine, 2023, Vol 12, Issue 7, p2472
- ISSN
2077-0383
- Publication type
Article
- DOI
10.3390/jcm12072472