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- Title
Intracellular Delivery of p53 Fused to the Basic Domain of HIV-1 Tat.
- Authors
Jiyoon Ryu; Hak Joo Lee; Kyeong-Ae Kim; Jae Yong Lee; Kil Soo Lee; Jinseu Park; Soo Young Choi
- Abstract
p53 is a potent tumor suppressor inactivated in many cancers. In this study, the membrane permeability of the HIV-1 Tat basic domain was exploited to introduce functional p53 into cancer cells. We expressed and purified a p53 fusion protein with the HIV-1 Tat basic domain at its N terminus (Tat-p53), and examined its transduction profile and biological activity in cancer cells. Tat-p53 was efficiently delivered to both the cytoplasm and nucleus of cells, and was transcriptionally active, as judged by the level of p21/WAF1 protein and of p21 promoter activity. Transduction of cells with Tat-p53 resulted in apoptotic cell death in both p53 positive and negative human tumor cell lines. These results suggest that Tat-p53 could be useful in cancer therapy.
- Publication
Molecules & Cells (Springer Nature), 2004, Vol 17, Issue 2, p353
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13050-0