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- Title
Activation of Mouse B Lymphocyte by Proteins Containing Hexahistidine.
- Authors
Ki Hwan Kim; Kyung Soo Kim; Choi, Sung E.; Jin-Kyoo Kim; Park, Jung-Hyun; Jae Hag Lee; Yup Kang
- Abstract
Many recombinant proteins are produced as a fusion protein tagging hexahistidine, which has been used for studying their biological function both in vitro and in vivo. Unexpectedly, we observed activation of BALB/c mouse splenocytes when treated with hexahistidine-tagged recombinant proteins. This activation was hexahistidine-specific since the anti-pentahistidine antibody completely neutralized the effect, and the bovine serum albumin conjugated with the hexahistidine peptides also showed a similar activation effect. The B cells seemed to be the activated splenocytes, since the cell population stained with the anti-immunoglobulin antibody and anti-CD80 antibody was increased after the treatment. However, the activation signal by hexahistidine was insufficient to fully differentiate the B lymphocytes. This result suggests that caution must be taken in the use of hexahistidine-tagged recombinant proteins, due to their nonspecific activation of B lymphocyte.
- Subjects
LYMPHOCYTES; RECOMBINANT proteins; RECOMBINANT molecules; B cells; SERUM albumin
- Publication
Molecules & Cells (Springer Nature), 2001, Vol 11, Issue 3, p287
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)17038-5