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- Title
Lactoferrin-inducible monocyte cytotoxicity for K562 cells and decay of natural killer lymphocyte cytotoxicity.
- Authors
McCormick, J. A.; Markey, G. M.; Morris, T. C. M.
- Abstract
Monocyte-enriched and lymphocyte-enriched fractions of peripheral blood from three healthy volunteers were obtained by percoll density gradient centrifugation. The cytotoxic activity of each fraction against 51Cr-labelled K562 cells was quantified in a 2-h assay using freshly isolated cells of each fraction and cells of each fraction which had been incubated with and without lactoferrin in complete medium for 18 h before performing the assay. We have thereby shown that cytotoxicity was not demonstrable in the lymphocyte fraction (containing 7 3 ± 0.4% large granular lymphocytes) after 18 h in medium, whereas the cytotoxicity of the monocyte fraction (containing 3 ± 0.04% large granular lymphocytes) was still significantly increased (P ⩽ 0.01) and that lactoferrin had no effect on lymphocyte fraction cytotoxicity while producing an U-fold increase in the cytotoxicity of the monocyte fraction. It is therefore possible to perform a relatively simple test of monocyte cytotoxicity using lactoferrin as a stimulant in a 2-h 51Cr-labelled K562 assay system by allowing 18 h to elapse for lymphocyte natural killer cytotoxicity to decay.
- Subjects
LACTOFERRIN; IRON proteins; MONOCYTES; LYMPHOCYTES; CELL-mediated cytotoxicity; CELL death
- Publication
Clinical & Experimental Immunology, 1991, Vol 83, Issue 1, p154
- ISSN
0009-9104
- Publication type
Article