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- Title
Recombinant 70--kD protein used for determination of autoantigenic epitopes recognized by anti--RNP sera.
- Authors
Nyman, U.; Lundberg, I.; Hedfors, E.; Pettersson, I.
- Abstract
A panel of high- and low-litre anti-RNP-positive patient sera was tested for reactivity with human snRNP proteins. The UI snRNP-specific 70-kD peptide was found to be the most prominent RNP antigen recognized by high-litre anti-RNP sera, mainly found in patients with mixed connective tissue disease (MCTD). The reactivity with the 70-kD protein was further analysed with recombinant fusion proteins containing different segments of the protein. One major and two minor antigenic regions were found. Most patient sera, both with high- and low-titre anti-RNP antibodies, only recognized the major region. The most aminoterminal region, showing partial sequence homology with a mouse retroviral p30 gag protein, contained an epitope that was recognized by one serum only.
- Subjects
MIXED connective tissue disease; CONNECTIVE tissue diseases; PROTEINS; BIOMOLECULES; BLOOD plasma; SERUM
- Publication
Clinical & Experimental Immunology, 1990, Vol 81, Issue 1, p52
- ISSN
0009-9104
- Publication type
Article