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- Title
A Stability Indicating Validated Method for the Determination of Lercanidipine Using Reverse Phase High Performance Liquid Chromatography.
- Authors
Mondal, Sumanta; Pushpalatha, Reddipalli; Mondal, Prasenjit; Mahapatra, Ashes Sinha; Shit, Dipankar; Das, Anik Kumar; Biswal, Sabyasachi
- Abstract
Objective: An accurate reverse phase High Performance liquid chromatography (RP-HPLC) method has been developed, validated and applied to stability indicating studies to determine Lercanidipine HCl in dosage form. Methods: Optimized chromatographic conditions were achieved by using Symmetry C18 Column, 250 mmx4.6 mm and 5μm particle size as stationary phase and Dihydrogen Orthophosphate Buffer: Methanol: ACN = (40 : 40 : 20) as eluent at flow rate 1.0 ml/min. UV detection was performed at 256nm. The developed method was validated and stability study was conducted as per ICH guidelines. Results: The retention time was found at 4.778 min. The method shows linearity over a range of 6 μg/ml to 40 μg /ml. The obtained correlation coefficient is 0.999. The LOD and LOQ values were 0.09 and 0.27 μg /ml. The acidic and alkaline stressed study shows more degradation of 8.181% and 7.241%. Conclusion: The present developed method was found stability indicating, easy and reliable method can be applied for routine analysis of lercanidipine in bulk drug and the Pharmaceutical formulations.
- Subjects
HIGH performance liquid chromatography; ULTRAVIOLET spectrophotometry; RF values (Chromatography); SUPERCRITICAL fluid chromatography
- Publication
International Journal of Pharmaceutical Investigation, 2020, Vol 10, Issue 2, p197
- ISSN
2230-973X
- Publication type
Article
- DOI
10.5530/ijpi.2020.2.36