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- Title
Heterologous Expression of a Membrane-Spanning Auxin Importer: Implications for Functional Analyses of Auxin Transporters.
- Authors
Carrier, David John; Bakar, Norliza Tendot Abu; Lawler, Karen; Dorrian, James Matthew; Haider, Ameena; Bennett, Malcolm John; Kerr, Ian Derek
- Abstract
Biochemical studies of plant auxin transporters in vivo are made difficult by the presence ofmultiple auxin transporters and auxininteracting proteins. Furthermore, the expression level of most such transporters in plants is likely to be too low for purification and downstream functional analysis. Heterologous expression systems should address both of these issues. We have examined a number of such systems for their efficiency in expressing AUX1 from Arabidopsis thaliana. We find that a eukaryotic system based upon infection of insect cells with recombinant baculovirus provides a high level, easily scalable expression system capable of delivering a functional assay for AUX1. Furthermore, a transient transfection system in mammalian cells enables localization of AUX1 and AUX1-mediated transport of auxin to be investigated. In contrast, we were unable to utilise P. pastoris or L. lactis expression systems to reliably express AUX1.
- Subjects
AUXIN; BIOLOGICAL transport; RECOMBINANT baculoviruses; ARABIDOPSIS thaliana; EUKARYOTIC cells
- Publication
International Journal of Plant Genomics, 2009, Vol 2009, p1
- ISSN
1687-5370
- Publication type
Article
- DOI
10.1155/2009/848145